Several rat embryonic stem cell lines developed by Qi-Long Ying (USC) as well as cell lines isolated in-house by the RRRC are available for distribution to interested investigators.  These ES cell lines represent an important reagent for developing genetically engineered rat models. 

The Resource Center has been working with these ES cell lines to further refine methods and protocols for establishing cultures and maintaining them.  In addition, we are generating rat knock-out models and isolating new ES cell lines from different inbred and outbred strains to provide additional tools for model development. 

Germline Transmisson of a Novel Rat Embryonic Stem Cell Line Derived from Transgenic rats. Men H. Bauer BA, Bryda EC. (2012) Stem Cells Dev.  21(14):2606-2612.

Derivation of a Germline Competent Transgenic Fischer 344 Embryonic Stem Cell Line. Men, H and Bryda, EC. (2013) PLoS One 8:e56518.

Efficient generation of selection-gene-free rat knockout models by homologous recombination in ES cells. Lan H, Li S, Guo Z, Men H, Wu Y, Li N, Bryda EC, Capecchi MR, Wu S. (2016) FEBS Lett. 590(19):3416-3424.